Prep HPLC Column, RP C4, Wide Pore, 10um, 300A. 21.2mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. The 300 Å wide pore structure provides improved access to the bonded phase for larger biomolecules, making this stationary phase particularly well suited for proteins, peptides, enzymes, and other high-molecular-weight compounds. The moderate hydrophobicity of the C4 ligand offers excellent recovery and chromatographic performance for biomolecule purification while reducing excessive retention often encountered with more hydrophobic phases.
68004-P21-1003M is a preparative HPLC column with dimensions of 21.2 mm ID x 100 mm length, packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The large internal diameter is designed for purification workflows where greater sample loading and fraction collection are required. The 100 mm length provides an ideal balance between throughput and chromatographic resolution, making it suitable for process development, compound isolation, and pilot-scale purification. Typical flow rates are approximately 15–40 mL/min depending on solvent conditions, system capabilities, and application requirements.
This preparative column is specifically intended for purification of proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules. The combination of wide pore architecture, fully endcapped C4 chemistry, and preparative dimensions provides excellent analyte recovery, loading capacity, and reproducible purification performance. A specialized preparative HPLC system capable of higher flow rates, larger injections, and fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP C4 Butyl Wide Pore stationary phase
300 Å pore size optimized for biomolecules
Fully double endcapped chemistry
Traditional butyl (C4) bonded phase
Designed for preparative HPLC purification
Excellent recovery of proteins and peptides
High sample loading capacity
10 µm particles for lower operating backpressure
Suitable for higher flow rates
Ideal for biomolecule isolation and purification
Typical Applications
Preparative HPLC purification
Protein purification
Peptide purification
Enzyme purification
Biomolecule isolation
Pharmaceutical research
Biotechnology research
Process development
Pilot-scale purification
Fraction collection
Specifications
Product Type: Preparative HPLC Column
Stationary Phase: RP C4 Butyl Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 21.2 mm ID x 100 mm
Length: 100 mm
Typical Flow Rate: 15–40 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Designed for purification rather than routine analytical HPLC
Intended for collection and isolation of purified compounds
Optimized for proteins, peptides, enzymes, and other biomolecules
Provides substantially higher sample loading capacity than analytical columns
Requires a preparative HPLC system capable of higher flow rates and fraction collection
Wide pore structure improves accessibility for large molecules
Special Order Item